Bacteria that are resistant to antibiotics can lead to severe, hard-to-treat wound infections. Image: CDC on Unsplash.

In order to combat bacterial wound infections, Empa researchers have developed cellulose membranes equipped with antimicrobial peptides. Initial results show that the skin-friendly membranes made of plant-based materials kill bacteria very efficiently.

If germs invade a wound, they can trigger a long-lasting infection that may fail to heal or even spread throughout the body, leading to life-threatening blood poisoning (sepsis), the researchers say. The problem of antibiotic resistance is becoming more and more widespread, particularly in complex wounds, as bacteria such as staphylococci have become resistant to what was once the miracle weapon of medicine, they add. Empa researchers have therefore developed cellulose membranes, with which these infections can be eliminated early on.

The team led by Empa researcher Katharina Maniura from the Biointerfaces lab in St. Gallen produced fine membranes from cellulose using electrospinning technology. The cellulose fibres with a diameter of less than one micrometre were spun into a delicate multi-layered, three-dimensional fabric. The membranes became particularly flexible and at the same time stable after the researchers had added the polymer polyurethane to the spinning process.

In order to achieve an antibacterial effect, the researchers designed multifunctional peptides – which, they say, can bind to cellulose fibers and exhibit antimicrobial activity. According to the scientists, peptides have several advantages compared to larger proteins: they are easier to produce and more stable than proteins, which react more sensitively to the chemical conditions in a wound.

If the cellulose membranes are treated with such a peptide solution, the fibre scaffold will become saturated with peptides. In cell culture experiments, the researchers then showed that the peptide-containing membranes are well tolerated by human skin cells. However, the cellulose membranes were a death sentence for bacteria such as staphylococci, which are often found in poorly healing wounds. "In bacterial cultures, over 99.99 percent of the germs were killed by the peptide-containing membranes," says Maniura.

In future, the antimicrobial membranes will be equipped with additional functions. "The peptides might, for instance, be functionalized with binding sites that enable the controlled release of further therapeutic substances," says Maniura.

Further Information

Prof. Dr. Katharina Maniura

Biointerfaces

Phone +41 58 765 74 47

[email protected]